Notes from the Field

Measuring the Pulse of the Ocean

February 11th, 2017 by Ryan Vandermeulen

Act 1: Blowin’ in the wind

At the unholy hour of 0400, I find myself on the aft deck of the world-class research vessel Falkor, bubbling with excitement stemming from a unique combination of four shots of espresso, generally being a morning person, and, most importantly, preparing to test an experimental device that I have put my blood, sweat, and tears into. As I bear-hug my newly collected bucket full of seawater on the rocking transit back to the wet lab, I take a moment to silently congratulate myself on the superb display of stamina and posture; it appears that my sea legs have finally decided to make an appearance. The sun will be coming up soon. It’s a Bob Dylan kind of day. Time to turn up the music and get to work.

Act 2: There ain’t no party like a plankton party

Ryan Vandermeulen, optical oceanographer, is testing this instrument at sea for the first time. The experimental “photosynthetron” (a.k.a. electro-squid 4000) serves as an incubation chamber for seawater samples. SOI/ Vandermeulen

In addition to being an eclectic lab DJ, one of my roles as a scientist aboard this righteously amazing research vessel is to investigate the rates of biological activity among microscopic communities within the ocean. The invitees to this epic microbial festival include bacteria, viruses, zooplankton, larval fish, and phytoplankton (algae). The photosynthetic members of this microscopic plankton community (PHYTO-plankton) are the base of a complex ocean food web, and have a large cascading ecological impact on the abundance and diversity of fisheries that we depend on. In addition, phytoplankton play a significant role in the global cycling of carbon, as well as the production of oxygen we breathe. Just like the plants in your garden, phytoplankton utilize carbon dioxide and micronutrients in the seawater – along with various light harvesting pigments which capture sunlight at different wavelengths – to create new cell bodies.

A byproduct of this magnificent life-giving process of photosynthesis is the production of oxygen, which we measure with great precision as a proxy for the amount of carbon that has been fixed into organic matter. The remaining (non-photosynthetic) members of this microbial party are not particularly polite dinner guests and have a tendency to exploit the kindness of our fearless photosynthetic community by eating the food that the phytoplankton have dutifully prepared, namely themselves (phytoplankton bodies) along with their subsequent secretion of dissolved organic matter (zooplankton are quite the sloppy eaters). We measure this microscopic massacre in the form of oxygen consumption, because, like us, these microbial heterotrophs need oxygen and organic matter that phytoplankton produce in order to live. Collectively, the ocean acts as one breathing organism as this process plays out, silently taking in copious amounts of carbon dioxide from the atmosphere and giving us oxygen that we breathe. This balance (or lack thereof) of carbon/oxygen uptake versus production lends us insight into the overall health or metabolic status of an ecosystem, in a way similar to taking the pulse of your body.

The Various light levels of the photosynthetron simulate different light conditions through the day, and the subsequent oxygen production/consumption is measured using optical sensors. SOI/ Vandermeulen

Act 3: Some telescopic insights into microscopic processes

One of the more confounding elements of studying oceanography is dealing with the fact that many measurements only tell us something about one very specific time and place in a very large and dynamic ocean. So, in addition to these experiments, we are monitoring the “color” of the ocean with continuous underway measurements of the reflectance of sunlight from the water. As sunlight hits the ocean, phytoplankton are capturing some of this light for photosynthesis, and they imperceptibly change the color of the water as light scatters back from the sea. Out in this near-barren desert of photosynthetic activity, we use a very precise radiometric measurement of over 100 wavelengths across the ultraviolet/visible spectrum to detect these subtle changes. As a NASA scientist, my other research life consists of using data from ocean-observing satellites that monitor changes in the color of the ocean over the entire globe every day. By understanding more about the link between biological activity that I’m measuring in the lab, the types of phytoplankton in the water, and how this changes the color of the ocean, we can refine how we monitor these processes from space, and thus increase our understanding of carbon cycle dynamics on a global scale. Just to re-iterate, we are literally monitoring the activity of microscopic organisms that are too small to see with the naked eye with a telescope orbiting our planet at over 15,000 mph to study climate scale processes. I think that’s pretty neat.

Act 4: Some microscopic insights into life, the universe, and everything

It’s been a long day, so I am going to wind down, grab my guitar, and serenade the sunset. They say if you sing to plants, they grow better, so there is some scientific merit to this ritual, mind you. Incidentally, our artist-at-sea, Kirsten, has inspired me through one of her onboard art + science seminars to try and take a more careful and purposeful look at the world around me. I will temporarily suspend the thought of Rayleigh scattering and radiative transfer equations as I watch the sun’s rays extend across the horizon. For now, I’ll just listen and observe.

Every Other Breath

February 9th, 2017 by Mónika Naranjo González

As an undergraduate student, Colleen Durkin had no idea what to specialize in, but looking at a seawater sample through a microscope during a field-trip to Friday Harbor Labs changed that forever. “I suppose I had used a microscope before, but I can’t remember it,” she shares. “All I know is that to this day, I can recall what that particular sample looked like, with all of its copepods and cells swimming around.” Colleen has never looked back: since then plankton has been the driver of both her career and curiosity from Undergraduate to Doctorate in Oceanography.

Colleen Durkin, Oceanographer, is studying particle size and distribution in order to relate it to the carbon cycle and the capacity of the ocean to store carbon. SOI/ Mónika Naranjo González


Diatoms are Colleen’s favorite phytoplankton, with good reason. Not only are they tiny yet incredibly complex, they are also beautiful organisms. Diatoms are capable of creating a glass shell, which bestows an almost fantastical quality upon them. “The glass has nano-scale structures,” says Colleen as she examines the image of a star-like cell. “They look like a work of design, like art.” Beyond their evident beauty, diatoms have a monumental impact on everyday life on planet Earth.

In order to synthesize silica (glass), diatoms carry out complex chemical processes, absorbing silicon available in the water. SOI/ Colleen Durkin

Take a Breath and Thank Plankton

One out of every five breaths taken by any (and every) life form on the planet comes from a diatom. Diatoms are a kind of phytoplankton: that is, microscopic plants drifting in all bodies of water. They carry out photosynthetic processes, ultimately producing oxygen in the air we breathe.

Half of the oxygen available to us has been produced by phytoplankton – this means that every other breath comes from microscopic organisms in the ocean. Getting to know them better, their processes, their current status, and how a changing climate will affect them is essential to us, because they are essential to life.

Although scientists have a general idea of the kinds of phytoplankton that can be found in different regions of the world at certain periods of the year, they do not yet have a complete understanding of phytoplankton communities’ full distribution. Furthermore, there is still much to be learnt about the chemical processes each planktonic group carries out. Falkor’s current expedition will help contribute to creating a baseline, connecting diversity with the processes each organism is responsible for. Humans are changing the Earth rapidly, and we need to be able to monitor and understand ocean communities before long-term changes occur.

The specific chemical processes carried out by phytoplankton still need to be determined and studied. This is a microscopic picture of a coccolithophore (left). Diatoms (right) form silica shells. The reason behind this is still to be determined. One theory relates to protection from predators. SOI/ Colleen Durkin

Sea to Space

This Sea to Space Particle Investigation will allow an unprecedented view of ocean processes by using tools both in the ocean and satellites in the sky. Looking at the ocean from specialized satellites it is possible to detect colors, which help inform the presence of certain types of particles drifting in the water. This expedition will provide key information connecting the satellites’ data capturing with real time in-situ measurements. As a result, experts will improve algorithms for these satellites, allowing for better global estimates of oceanic biochemical processes.

Phytoplankton is a major foundation to nearly all known forms of life. It produces oxygen, regulates the climate and sustains the marine food chain – a fundamental source of protein for humans. At the same time, when these organisms sink, they drag carbon down to the depths of the oceans with them, counteracting the accumulation of carbon dioxide in the atmosphere. This is Colleen’s primary interest: carbon export from the air to the ocean. She knows she will never grow tired of studying phytoplankton, immersing herself in the fascinating chemical labyrinth that these creatures build – often out of sight.

Zen and the Art of Wirewalker Maintenance

February 3rd, 2017 by Melissa Omand

Melissa Omand, physical oceanographer, collaborated with Kirsten Carlson, Artist at Sea participant, to create a watercolor explaining the linkage involved in the Wirewalker’s whole assemble.

I have sailed with my share of compelling characters since I began ocean research, and perhaps it is fitting that while we study ocean color aboard the R/V Falkor, I have had the pleasure of getting to know one of the most colorful. Kirsten Carlson has joined the cruise as an artist-in-residence, bringing her zany, insightful perspectives and boundless energy to coax us from our laptops. Last night she encouraged me to try out her Artscipaedia Plankton coloring booklet. That is right – plankton – as in the microscopic organisms that color the ocean… exactly what the scientists are studying onboard. As it turns out, Kirsten has a background in marine science, and is a self-described plankton rescuer!

Colorful Shipmates

While coloring, I found my thoughts drawn back to the instrumentation I had released over the side the day before. My Wirewalker and sedimentation traps were miles away from our ship, drifting in the open ocean, collecting data and doing their thing… I hoped. I decided to draw the Wirewalker instrument array. I have spent months preparing for this cruise – testing the sensors, communications, preparing the sediment tubes, and making sure each piece of hardware is accounted for. I discovered I knew it by heart. Kirsten shared her watercolor paper and we devised a plan to collaborate – I would draw and she would paint. I can’t recall finding such catharsis on a cruise before. As the rest of our science party started a movie in our de facto hang-out space – alibrary/office/lounge/video conference room that has quickly become the center of orbit onboard the ship – I did my best to dust off an artistic identity I’ve rarely assumed since I was a visual art major in in high school.

Beauty in Buoys and Shackles

Drawing the mundane but critical details of each linkage in the array, I newly appreciated the elegant design of the Wirewalker: a design evolution I had observed since shortly after its invention by Dr. Rob Pinkel’s group at Scripps Institution of Oceanography. The Wirewalker is assembled in distinct segments. At the top, is a GPS-tracked buoy, and beneath this hangs a 3/16” stranded wire that stretches 150 m beneath the surface. As the buoy tugs the wire up and down over the waves, the sensor-laden Wirewalker uses a simple cam mechanism to rectify the wave action into a one-way motion down the wire. When it reaches the bottom, it hits a stop and releases it’s grip, gliding freely along the wire to the surface. This cycle is repeated every 10 minutes or so, giving us a remarkable high resolution view into the physical and physiological variations of the plankton and particles in the upper ocean.

Having a professional artist on-board (in addition to a number of talented crew and fellow scientists) has been a unique experience and has inspired me to reconnect with my inner artist. As I write this, I only hope that our collaborative art is an homage, and not an obituary to my lonely Wirewalker adrift amongst the enormous waves of the wintery North Pacific Ocean.

Melissa Omand will be working with Meg Estapa and Colleen Durkin to study the data collected by the Wirewalker’s assemble in order to understand Carbon Pump in these waters.

Microbes Run the Show

February 2nd, 2017 by Mónika Naranjo González

Researchers in the Dry Lab are able to examine the most recent Virtual Reality simulations created from holographic photos taken in each CTD cast. With this experience, it is clear that every single drop of water in the ocean contains huge amounts of life. Microscopic organisms constantly carry out processes that maintain the atmosphere, air, food webs, and life as we know them. Yet humans are changing things quickly, and we do not have enough knowledge to determine what our future world may look like. Hugo Berthelot, biogeochemical oceanographer, is on Falkor to fill in some of the blanks.

Hugo Berthelot, biogeochemical oceanographer, is on Falkor studying phytoplankton organisms and the biochemical processes they take part in.

Who is Doing What?

One crucial aspect of microbial communities that researchers want to understand can be summed up in the cheeky question “Who is doing what?” This is important to understand because of the delicate connections between all marine and terrestrial life, granting us a start to inferring the microscopic “winners” and “losers” in the face of Climate Change.

To do so, Hugo is studying the Nitrogen Cycle. Nitrogen is a key nutrient at the very base of the food chain, both on land and in the ocean. Its availability directly impacts the global marine ecosystem. Many microorganisms feast, grow, and live off of it. Once the microorganisms reach a certain size (or die), they will sink, dragging carbon – in the shape of organic matter – down with them. Once carbon leaves the superficial layers of the ocean and enters deep waters, it will be gone for thousands or even millions of years. As it stands today, a healthy functioning carbon sink is probably good news. This carbon pump has been working for thousands of years, helping regulate the Earth’s chemistry and temperature.

Understanding how available Nitrogen is for microbes to feed on, along with how different organisms are able to transform Nitrogen after they have ingested it, directly relates to the inner-workings of the biological carbon pump, Hugo’s main focus of research. He will collect water samples in very different geographical locations and weather conditions as Falkor sails from Hawaii to Portland, Oregon.

Invisible Gases and Microscopic Beings

Phytoplankton is very, very small. Studying which microscopic organisms transform an invisible gas (such as Nitrogen) and their rates at doing so is extremely challenging. Therefore, Hugo utilizes a special technology called nanoSIMS, but before he gets to that point, he must follow certain steps.

Using an incubator, Hugo Berthelot enriches the water with Nitrogen and exposes the microorganisms to this environment for 24 hours, to later assess how much Nitrogen each cell consumed.

He first collects water samples from the surface and from Niskin bottles deployed in CTD casts at the Deep Chlorophyll Maximum depth of 150 meters. The, using an incubator, he enriches the water with a stable form of Nitrogen (N15) while maintaining the microbial communities natural conditions, including light and temperature. After a day of incubation, he filters the water and freezes the samples, hence preserving each organism. Once back ashore, flow cytometry cell sorting will enable him to identify each kind of cell and sort them into taxonomic groups. Then nanoSIMS technology will detect the amount of N15 consumed by each cell. After months of rigorous and complex analysis, Hugo will be closer to understanding which organisms are carrying out certain chemical processes, as well as which ones are likely to benefit from changes in global climate.

Hugo freezes the filtered samples using liquid Nitrogen. After leaving Falkor, he will analyze the cells to understand which chemical processes each one carries out and how they responded to the incubator experiment.

Every microorganism specializes in one single task, transforming one chemical element into another and fuelling our planet’s biochemical system. Therefore, changes in microbial populations will inevitably affect Earth’s chemistry. Once the snowball of a changing climate has begun rolling, it will trigger other factors – such as changes in oceanic microscopic ecology – which will in turn add to the snowball’s speed and impact. Figuring out what life inside a drop of seawater may look like in the future is essential knowledge for researchers to understand what the future may hold.

Science Challenges at Sea: A Plumbing Story

January 31st, 2017 by Seaver Wang, PhD student, Duke University

This is my instrument, which is designed to measure dissolved gases in seawater to detect the oxygen produced by algae. Sea Sparkle, my trusty green stuffed plankton assistant, is keeping a careful eye on the data we’re collecting!

When people think of science, the first examples that come to mind are often dramatic triumphs—the moon landing, the invention of DNA sequencing, or the discovery of the polio vaccine. Almost every exciting experimental result, however, is the result of an enormous amount of unseen effort. Thorough preparation, resourceful improvisation, success and failure, and improvement through trial and error are all deeply familiar to any researcher!

At sea on the Falkor, the challenges that we face as a part of ocean science are unique. After all, any unanticipated problems must be solved using only the resources on board our two-hundred-foot vessel.

Our first two days at sea have presented their fair share of challenges. Our cruise plans have changed almost daily in order to adapt to weather patterns and the ship’s limits in terms of speed and range. In the “wet” lab, anywhere from six to eight science team members must work elbow-to-elbow with all of their equipment packed into a space little larger than an examination room at the doctor’s office.

For my research, which involves measuring dissolved gases from seawater to detect plankton activity, plumbing has been my biggest enemy so far.

The seawater I am measuring is piped continuously aboard ship via a pump, and this water is available from only two outlets in the already-crowded lab. My original plan to use seawater from one of the two faucets was scuppered when a crucial valve broke, spraying the lab with ocean water like a fire hose! After shutting the faucet and confirming that none of our sensitive electronic instruments had been damaged, we realized that I would now have to take my water from the second outlet—located on the opposite side of the room from where I had set up my seawater supply tubing…! In addition to reorganizing all of my plumbing, I would also have to find materials to connect to the second faucet, which uses a different connection from the first.

Luckily, scientists can be pretty inventive! My seawater supply pipe was moved to hang from the ceiling in order to cross the room, and I borrowed parts from other members of the science team in order to fit my tubing to the new faucet. Sharing the lab space is also an important part of ocean science, and so Ryan, another scientist on board, graciously moved some of his equipment to give me access to the faucet.

When doing science at sea, it’s also important to learn from mistakes and bad luck quickly. This time, we fitted double metal clamps to the faucet connection to reduce the risk of unleashing a second fire hose event. With that final step, my plumbing was finally usable, allowing me to switch on my instrument and start collecting my valuable data!

In the end, we spent several hours fixing this incredibly boring problem—one which has very little to do with actual science. Paradoxically, however, all of our tinkering with tubes and faucets was essential in order to perform my research. This is just one of the many small challenges that we have dealt with as we prepare for one of the busiest parts of our expedition. Even though many of our problem-solving stories will never be mentioned when we publish our research results, they are just as much of a part of our science as designing experiments or picking locations for the Falkor to explore.

It’s little challenges like this that help me remember that every scientific discovery, great or small, is built on a huge foundation of invisible work!